Bio::Restriction::Enzyme::MultiSite - A single restriction endonuclease
# set up a single restriction enzyme. This contains lots of
# information about the enzyme that is generally parsed from a
# rebase file and can then be read back
This module is used for restriction enzymes that recogonize more than one site.
There are some enzymes that recognize sites that cannot be represented by the
ambiguous genetic code. For example, M.PhiBssHII recognizes the sites:
ACGCGT,CCGCGG,RGCGCY,RCCGGY, and GCGCGC
Each site gets its own object that Bio::Restriction::Enzyme will refer to. Each
also correlates with the other sites using the method others which stores
references to other objects with alternative sites.
In this schema each object within an EnzymeCollection can be checked for
matching a sequence.
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modules. Send your comments and suggestions preferably to one of the Bioperl
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include a thorough description of the problem with code and data examples if
at all possible.
Report bugs to the Bioperl bug tracking system to help us keep track the bugs
and their resolution. Bug reports can be submitted via the web:
Heikki Lehvaslaiho, heikki-at-bioperl-dot-org
Rob Edwards, firstname.lastname@example.org
Copyright (c) 2003 Rob Edwards.
Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All Rights
Reserved. This module is free software; you can redistribute it and/or modify
it under the same terms as Perl itself.
Methods beginning with a leading underscore are considered private and are
intended for internal use by this module. They are not considered part of the
public interface and are described here for documentation purposes only.
Title : new
Function : Initializes the enzyme object
Returns : The Restriction::Enzyme::MultiSite object
Title : others
Usage : $re->others(@others);
Function : Gets/Sets the a list of other sites that this enzyme recoginizes
Arguments : An array containing the other Bio::Restriction::Enzyme::MultiSite
Returns : An array containing the other Bio::Restriction::Enzyme::MultiSite
Title : purge_others
Usage : $re->purge_references();
Function : Purges the set of references for this enzyme